Enabling Technologies for NAMs
Advanced in vitro models are adopted because they retain physiology that conventional culture discards. That same complexity creates two practical constraints: complex models are difficult to interrogate, and difficult to control. Our Enabling Technologies address both. We engineer the biological content of a model so it becomes readable, and we configure and characterize the physical environment it sits in so that what is read can be attributed to the test article. Each is available as a standalone service, and each is deployed internally when we build evaluation studies.
Integrated Technical Capabilities
Stable Lentiviral Transduction of 3D Tumor Microtissues
Making Complex Tumor Models Genetically Readable
Transient methods penetrate dense matrix poorly and give only short-term expression. Lentiviral integration is heritable, so the modification persists through passaging and reaches both dividing and non-dividing cells, which matters in patient-derived material.
We establish stable engineered lines - reporters, knockdown, knockout, and overexpression constructs - with protocols developed per tissue origin, delivered as polyclonal pools or clonally derived lines with a documented QC report.
Typical Questions It Answers
Explore Stable Lentiviral Transduction of 3D Tumor MicrotissuesDynamic Perfusion Assay Configuration Services
Controlling the Biophysical Environment the Assay Depends On
A predictive microphysiological system requires more than culturing cells in a vessel. Flow profile, matrix properties, and substrate material all shape the readout, and a consumable that absorbs the test article produces a dose-response curve describing the plastic rather than the compound.
We configure commercially available perfusion platforms against the intended endpoint: simulation-led parameter selection, substrate choice matched to the assay rather than defaulted, and baseline validation to rule out material-induced artifacts. Delivered with a technical data package covering fluidic, matrix, and material characterization.
Typical Questions It Answers
Explore Dynamic Perfusion Assay Configuration ServicesHow These Work Together
Engineered biology and controlled biophysics are independently useful, and can be combined where a program needs a longitudinally readable model under characterized conditions.
| Engineered Biology | Controlled Biophysics | |
|---|---|---|
| Service | Stable Lentiviral Transduction | Dynamic Perfusion Assay Configuration |
| Question Addressed | Can the model report what we need to measure? | Is the measurement attributable to the test article? |
| Delivered as | Cryopreserved engineered lines with QC report | Transferable protocol with technical data package |
Working with ProNAMs
Developed per system, not applied generically. Protocol parameters are established for each tissue origin; platform parameters are selected against the intended endpoint.
Feasibility before commitment. Both services include an explicit feasibility stage. Where results indicate the approach will not hold, scope and timeline are revised and re-confirmed before work continues.
Documented, not asserted. Deliverables include the underlying data, so reported results are traceable to measurements.
Downstream continuity. Outputs can be carried directly into our in vitro NAMs evaluation services, or delivered for use in your own workflows.
Start a Technical Assessment
Tell us the biological question and the endpoint it has to support. We will advise on model engineering requirements, platform configuration, feasibility, and indicative timeline.
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